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Confocal laser scanning microscope

Confocal microscopes are used in medical as well as cosmetic research

This technology can be applied for skin cancer, in cases of burns, dermatitis, and for the cosmetics research industry.

How to use a confocal microscope

Contrary to a biopsy which consists of excising a tissue sample, confocal laser scanning microscopy is a non-invasive procedure, i.e. a procedure that does not penetrate or damage skin. The process is completely pain-free and delivers images of living tissue in real-time.

p>After applying a liquid (water or oil), a laser beam is directed to the skin region to be examined where it is then reflected.

The confocal arrangement of the illuminated skin region, the light source, and the aperture opening of the detector makes it possible to generate images of a clearly defined thin layer of skin. The image contrast is created by the different indexes if refraction of the skin components.

This gentle examination method does not have any negative side effects. This means highly sensitive skin areas such as is the case with an acute contact allergy can be examined and assessed without discomfort to the patient.

Microscope usage

The dermatologist simply attaches an adhesive window to the stainless steel tissue ring of the device, applies oil onto the window, and then places the window onto the skin.

Microscope usage

After starting the device, the dermatologist starts the examination by generating a dermatoscopic image using the VivaCam.

Microscope usage

The dermatoscopic image is correlated to the confocal image and may be used to navigate the laser in the lesion.

Microscope usage

To generate a confocal image, a small amount of ultrasound gel is applied to the center of the adhesive window.

Microscope usage

The laser tube is affixed to the tissue ring and the VivaScope 1500 starts taking an image.

Microscope usage

Different sets of confocal images can be acquired as desired.

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